abstract：:Metal tolerance has been found to vary among Acidithiobacillus ferrooxidans strains and this can impact the efficiency of biomining practices. To explain observed strain variability for differences in metal tolerance we examined the effects of Cu(2+) and Ni(2+) concentrations (1-200 mM) on cytoplasmic membrane propert...

journal_title：Microbiological research

authors： Mykytczuk NC,Trevors JT,Ferroni GD,Leduc LG

更新日期：2011-03-20 00:00:00

abstract：:The genus Enterococcus belong to the genera of bacteria that produce lactic acid and can confer health benefits to living organisms. Selenium (Se) is an essential micronutrient for humans and animals. Thirty-six Enterococcus species isolated from dairy products were screened for Se(IV) sorption capacity for use as a p...

journal_title：Microbiological research

authors： Pieniz S,Okeke BC,Andreazza R,Brandelli A

更新日期：2011-03-20 00:00:00

abstract：:Pythium intermedium is known to play an important role in the carbon cycling of cool-temperate forest soils. In this study, a fast, precise and effective real-time PCR technique for estimating the population densities of P. intermedium from soils was developed using species-specific primers. Specificity was confirmed ...

journal_title：Microbiological research

authors： Li M,Senda M,Komatsu T,Suga H,Kageyama K

更新日期：2010-10-20 00:00:00

abstract：:The severe and fatal human disease, tularemia, results from infection with the Gram-negative pathogen Francisella tularensis. Identification of surface outer membrane proteins, specifically lipoproteins, has been of interest for vaccine development and understanding the initiation of disease. We sought to identify Fra...

journal_title：Microbiological research

authors： Parra MC,Shaffer SA,Hajjar AM,Gallis BM,Hager A,Goodlett DR,Guina T,Miller S,Collins CM

更新日期：2010-09-20 00:00:00

abstract：:Alcaligenes sp. strain O-1 inducibly deaminates 2-aminobenzenesulfonate (ABS) via dioxygenation to 3-sulfocatechol, which is desulfonated during meta ring-cleavage to yield 2-hydroxymuconate. This intermediate is transformed through the oxalocrotonate-branch of the sulfocatechol meta-pathway (Scm). The complete pathwa...

journal_title：Microbiological research

authors： Ruff J,Smits TH,Cook AM,Schleheck D

更新日期：2010-05-30 00:00:00

abstract：:The SAP6 gene (without signal sequence) encoding Metschnikowia reukaufii acid protease was amplified by PCR and fused to the expression vector pET-24a(+). The carboxy-terminal 6x His-tagged recombinant acid protease (rSAP6) was expressed from pET-24a(+)SAP6-6His in Escherichia coli BL21 (DE3) and purified with affinit...

journal_title：Microbiological research

authors： Li J,Chi Z,Wang X

更新日期：2010-03-31 00:00:00

abstract：:Mycobacterium avium subspecies paratuberculosis (MAP), the causative agent of Johne's disease (JD) in animals, has also been linked with Crohn's disease in human beings. Lack of indigenous diagnostics and vaccine hampered control of JD in India. Designing effective control strategies require thorough understanding of ...

journal_title：Microbiological research

authors： Sohal JS,Singh SV,Singh PK,Singh AV

更新日期：2010-02-28 00:00:00

abstract：:Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) expressing Panton-Valentine leukocidin (PVL) have been characterized worldwide, but it has not been done in Egypt. In this study, we analyzed the molecular characteristics of PVL+CA-MRSA in Egypt, compared their genetic patterns with that of PVL+...

journal_title：Microbiological research

authors： Enany S,Yaoita E,Yoshida Y,Enany M,Yamamoto T

更新日期：2010-02-28 00:00:00

abstract：:Human interferon alpha2b (IFN-alpha2b) is a pleiotropic cytokine used for the treatment of various cancers. Antibacterial peptide CM4 is a small peptide that can strongly inhibit many kinds of bacteria, fungi, and tumor cells, but it does no harm to normal cells. Here, we describe a protein expression system for the p...

journal_title：Microbiological research

authors： Li NN,Liu P,Chen SJ,Lin QP,Zhou LF,Zhang SQ

更新日期：2010-02-28 00:00:00

abstract：:Prolonged cold storage of raw milks favors the growth of psychrotrophs, which produce heat-resistant exoenzymes of considerable spoilage potential; the bacterial proteases and lipases affect raw milk quality; among them phospholipases (PLs) may target the milk fat globule. More importantly, bacterial PLs are key virul...

journal_title：Microbiological research

authors： Munsch-Alatossava P,Gursoy O,Alatossava T

更新日期：2010-01-01 00:00:00

abstract：:Twenty-nine staphylococcal isolates from different clinical samples were tested for slime production: phenotypic characterization was carried out using Christensen test (tube test) and Congo red agar plate test (CRA plate test), while the presence and expression of icaA and icaD genes were evaluated by real-time PCR. ...

journal_title：Microbiological research

authors： Liberto MC,Matera G,Quirino A,Lamberti AG,Capicotto R,Puccio R,Barreca GS,Focà E,Cascio A,Focà A

更新日期：2009-01-01 00:00:00

abstract：:Sponges harbor diverse prokaryotic and eukaryotic microbes. However, the nature of sponge-fungal association and diversity of sponge-derived fungi have barely been addressed. In this study, the cultivation-dependent approach was applied to study fungal diversity in the Hawaiian sponges Gelliodes fibrosa, Haliclona cae...

journal_title：Microbiological research

authors： Li Q,Wang G

更新日期：2009-01-01 00:00:00

abstract：:Aerated lagoons are commonly used for domestic and industrial wastewater treatment due to their low cost and minimal need of operational requirements. However, little information is known regarding microbial communities that inhabit these ecosystems. In this study, a 16S-DGGE approach was used to estimate bacterial di...

journal_title：Microbiological research

authors： Moura A,Tacão M,Henriques I,Dias J,Ferreira P,Correia A

更新日期：2009-01-01 00:00:00

abstract：:The BIOLOG system was used to generate knowledge of enterococci populations found in fresh and dry manure of livestock (cattle (Bos taurus), horse (Equus caballus), and sheep (Ovis aires)). Six-hundred and forty Enterococcus isolates from the host sources were observed as a combined fresh manure unit and a combined dr...

journal_title：Microbiological research

authors： Graves A,Weaver RW,Entry J

更新日期：2009-01-01 00:00:00

abstract：:The pathogenicity of a new China variety of Metarhizium anisopliae (M. anisopliae var. dcjhyium) against the subterranean termite Odontotermes formosanus and the effect of the fungal fermented solution on hemolymph intracellular calcium were studied in laboratory. Conidia from the M. anisopliae var. dcjhyium were high...

journal_title：Microbiological research

authors： Dong C,Zhang J,Huang H,Chen W,Hu Y

更新日期：2009-01-01 00:00:00

abstract：:Vibrio vulnificus is a serious bacterial pathogen for humans and aquatic animals. We developed a rapid, sensitive and specific identification method for V. vulnificus using loop-mediated isothermal amplification (LAMP) technique. A set of primers, composed of two outer primers and two inner primers, was designed based...

journal_title：Microbiological research

authors： Ren CH,Hu CQ,Luo P,Wang QB

更新日期：2009-01-01 00:00:00

abstract：:Hirsutella rhossiliensis OWVT-1 has substantial potential as a biocontrol agent against plant-parasitic nematodes. Serine proteases have emerged as a potentially useful factor in the nematode-fungus interactions. When grown in liquid culture with the nematode Panagrellus redivivus as the sole nitrogen source, an extra...

journal_title：Microbiological research

authors： Wang B,Liu X,Wu W,Liu X,Li S

更新日期：2009-01-01 00:00:00

abstract：:Hydrogenases are the key enzymes for the biological hydrogen production, which can be classified as H(2)-uptake hydrogenase and H(2)-production hydrogenase. The genes encoding a membrane-bound [NiFe]-hydrogenase (MBH), which is mainly responsible for hydrogen uptake, from the photosynthetic bacterium Allochromatium vi...

journal_title：Microbiological research

authors： Liang Y,Wu X,Gan L,Xu H,Hu Z,Long M

更新日期：2009-01-01 00:00:00

abstract：:In spite of reports on cytokines induction by the Brucella DNA in murine model, there is no comparison between pathogenic and appropriate vaccine strains in human. We investigated the cytokines profile of human peripheral blood mononuclear cells (PBMCs) induced by DNA extracted from pathogenic isolates of Brucella mel...

journal_title：Microbiological research

authors： Lashkarbolouki T,Ardestani SK,Kariminia A,Ziaee AA,Torkabadi E,Ebrahimi M

更新日期：2008-01-01 00:00:00

abstract：:Among the 49 strains of moderately halophilic bacteria isolated from the salty environments of Iran, a Gram-positive coccus designated as strain QW6 showed high capacity in the removal of toxic oxyanions of tellurium in a wide range of culture medium factors including pH (5.5-10.5), temperature (25-45 degrees C), vari...

journal_title：Microbiological research

authors： Amoozegar MA,Ashengroph M,Malekzadeh F,Reza Razavi M,Naddaf S,Kabiri M

更新日期：2008-01-01 00:00:00

abstract：:The survivability of Salmonella cells in popcorn preparation was determined for two distinct cooking methods. The first method used a standard microwave oven. The second method used conventional cooking in a pan. Prior to thermal processing in independent experiments, 12 suspensions in a range between 1x10(3) and 8x10...

journal_title：Microbiological research

authors： Anaya I,Aguirrezabal A,Ventura M,Comellas L,Agut M

更新日期：2008-01-01 00:00:00

abstract：:Foxing of paper is a deterioration phenomenon occurring in the form of brown-yellowish spots, the abiotic and/or biotic causes of which are not yet completely understood. Nevertheless, microbiological infection has been recognized that may contribute to paper damage and therefore it becomes important to know the taxon...

journal_title：Microbiological research

authors： De Paolis MR,Lippi D

更新日期：2008-01-01 00:00:00

abstract：:Polyamines are small polycations essential for cell growth. Ornithine decarboxylase (ODC), the first enzyme of the polyamine synthesis pathway, is one of the most regulated enzymes of eukaryotes. In the present report, the transcriptional regulation of the gene encoding ODC from the dimorphic fungus Yarrowia lipolytic...

journal_title：Microbiological research

authors： Jiménez-Bremont JF,Ruiz-Herrera J

更新日期：2008-01-01 00:00:00

abstract：:Twenty-nine rhizobial isolates from root nodules of the wild Legumes Astragalus, Lespedeza and Hedysarum growing in the northwestern region of China, were characterized by numerical taxonomy, RFLP and sequencing of PCR-amplified 16S rDNA genes, and cross-nodulation with selected Legume species. Based on the results fr...

journal_title：Microbiological research

authors： Wei GH,Zhang ZX,Chen C,Chen WM,Ju WT

更新日期：2008-01-01 00:00:00

abstract：:Saccharomyces cerevisiae and Candida shehatae were co-immobilized in an agar sheet which was introduced in an original two-chambered bioreactor asymmetrically fed in a batch mode with a mixture of glucose and xylose in a ratio of 35:15. The two sugars were consumed simultaneously. All glucose was fermented but only 20...

journal_title：Microbiological research

authors： Lebeau T,Jouenne T,Junter GA

更新日期：2007-01-01 00:00:00

abstract：:There is a growing interest to find alternate bioresources for production of ethanol, apart from cane/sugar beet molasses and starchy crops like sweet sorghum, cassava and sweet potato. Mahula (Madhuca latifolia L.) is a forest tree abundantly available in the Indian subcontinent and its flowers are very rich in ferme...

journal_title：Microbiological research

authors： Swain MR,Kar S,Sahoo AK,Ray RC

更新日期：2007-01-01 00:00:00

abstract：:We have now entered in the post-genomic era, where we have knowledge of plethora of fungal genomes and cutting edge technology is available to study global mRNA, protein and metabolite profiles. These so-called 'omic' technologies (transcriptomics, proteomics and metabolomics) provide the possibility to characterize p...

journal_title：Microbiological research

authors： Bhadauria V,Popescu L,Zhao WS,Peng YL

更新日期：2007-01-01 00:00:00

abstract：:Tannase producing fungal strains were isolated from different locations including garbages, forests and orchards, etc. The strain giving maximum enzyme yield was identified to be Aspergillus ruber. Enzyme production was studied under solid state fermentation using different tannin rich substrates like ber leaves (Zyzy...

journal_title：Microbiological research

authors： Kumar R,Sharma J,Singh R

更新日期：2007-01-01 00:00:00

abstract：:The saline pond microalga, Dunaliella salina (Dunal) Teod. maintained in De Walne's (basal) medium under laboratory conditions was confirmed by amplifying the chromosomal DNA of the microalga by PCR with specific primers MA1 and MA2. Seaweed extracts obtained from Sargassum wightii and Ulva lactuca were amended separa...

journal_title：Microbiological research

authors： Raja R,Hema Iswarya S,Balasubramanyam D,Rengasamy R

更新日期：2007-01-01 00:00:00

abstract：:To evaluate the roles of blaIMP and blaTEM genes in the resistance of Serratia marcescens against beta-lactams and to find the spreading ways of these genes, 19 clinical isolates of imipenem-resistant Serratia marcescens were analyzed. Six strains bore blaIMP and blaTEM genes on a single plasmid, as confirmed by trans...

journal_title：Microbiological research

authors： Zhao WH,Hu ZQ,Chen G,Matsushita K,Fukuchi K,Shimamura T

更新日期：2007-01-01 00:00:00

abstract：:Colonization of micropropagated sugarcane plants by Gluconacetobacter diazotrophicus and Herbaspirillum sp. was confirmed by a dot-immunoblot assay. In all, a 45-day short-term and 180-day long-term experiments conducted on micropropagated sugarcane plants of Co 86032, a sugar rich popular variety in South India, indi...

journal_title：Microbiological research

authors： Muthukumarasamy R,Govindarajan M,Vadivelu M,Revathi G

更新日期：2006-01-01 00:00:00

abstract：:We have cloned a laccase gene fragment isolated from a Trametes versicolor strain in Korea. It showed high similarity in nucleotide sequences when compared with other fungal laccases. TNT (2,4,6-trinitrotoluene), a widely used explosive, was transformed rapidly by T. versicolor. When TNT and its catabolic intermediate...

journal_title：Microbiological research

authors： Cheong S,Yeo S,Song HG,Choi HT

更新日期：2006-01-01 00:00:00

abstract：:A comparative study was conducted of a novel real-time quantitative PCR test (LightCycler System) with FastStart DNA Master(PLUS) SYBR Green I dye to detect DNA of human herpes virus 6 (HHV-6). Results were compared with those of a real-time quantitative PCR with hybridization probe (HP) formats using the fluorescence...

journal_title：Microbiological research

authors： Fernández F,Gutiérrez J,Sorlózano A,Romero JM,Soto MJ,Ruiz-Cabello F

更新日期：2006-01-01 00:00:00

abstract：:Ammonia production from urea by ureolytic oral bacteria is believed to have a significant impact on oral health and ecological balance of oral microbial populations. Actinomyces naeslundii is an important ureolytic organism in the oral cavity. In this study, we aimed to investigate the substrate affinity and pH optimu...

journal_title：Microbiological research

authors： Liu Y,Hu T,Zhang J,Zhou X

更新日期：2006-01-01 00:00:00

abstract：:Methicillin-resistant staphylococci may also be resistant to some other antibiotics as well as beta-lactams. In this study, co-existence of resistance to methicillin and aminoglycosides was genetically investigated in staphylococci. A total of 50 staphylococci from in-patients, 17 Staphylococcus aureus and 33 coagulas...

journal_title：Microbiological research

authors： Ardic N,Sareyyupoglu B,Ozyurt M,Haznedaroglu T,Ilga U

更新日期：2006-01-01 00:00:00

abstract：:Bacterial utilization of monomers is recognized as an important step in the biogeochemical cycling of organic matter. In this study we have compared the heterotrophic activity of bacterial communities from different micro-habitats within a salt marsh environment (Ria de Aveiro, Portugal) in order to establish spatial ...

journal_title：Microbiological research

authors： Cunha MA,Pedro R,Almeida MA,Silva MH

更新日期：2005-01-01 00:00:00

abstract：:Extended spectrum beta-lactamases (ESBLs) usually associated with multiple drug resistance, including beta-lactam and non-beta-lactam antibiotics. This resistance can cause Limitation in the choice of drugs appropriate for using in clinical practice, especially in life-threatening infections. In this study we aimed to...

journal_title：Microbiological research

authors： Kizirgil A,Demirdag K,Ozden M,Bulut Y,Yakupogullari Y,Toraman ZA

更新日期：2005-01-01 00:00:00

abstract：:Systemic yeast infections are the Leading cause of mortality and morbidity in immunocompromized patients. Candida albicans, being the most frequently isolated fungal pathogen in these patients, can be divided into three genotypes (genotypes A, B and C) by 25S intron analysis. In our study, we found that molecular size...

journal_title：Microbiological research

authors： Karahan ZC,Akar N

更新日期：2005-01-01 00:00:00

abstract：:Bacteria of the genus Agrobacterium can transfer a portion of their Ti plasmid (T-DNA) in complex with the VirE2 and VirD2 proteins into the plant-cell nucleus and cause it to be integrated in the host-cell chromosomes. The mechanism of T-DNA transfer across the plant-cell membrane and cytoplasm is unknown. The aim of...

journal_title：Microbiological research

authors： Volokhina I,Sazonova I,Velikov V,Chumakov M

更新日期：2005-01-01 00:00:00

abstract：:The pectinolytic enzyme from the solid-state culture of Rhizopus oryzae NBRC 4707 was purified to homogeneity by column chromatography on CM-Toyopearl 650 M and hydroxylapatite. The molecular weight of the enzyme was estimated by SDS-polyacrylamide gel electrophoresis to be 31,000 and was reduced to 29,700 after treat...

journal_title：Microbiological research

authors： Saito K,Takakuwa N,Oda Y

更新日期：2004-01-01 00:00:00